INTRODUCTION

Glucagon is a peptide hormone that increases blood glucose levels via release of liver glycogen stores. A robust and precise physicochemical chromatographic procedure is used in the glucagon assay to assign potency on a mass basis. Bioidentity is still required in Glucagon for Injection, and a procedure for the production of cyclic adenosine monophosphate (cAMP) in vitro, in response to glucagon stimulation of the glucagon receptor cell line, is presented here.