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〈1023〉 Evaluation Strategy for Trace Elements in Cell Culture Media Used in the Manufacture of Recombinant Therapeutic Proteins

ELEMENTS OF INTEREST

Cell culture media [which includes basal cell culture media, nutrient feed, and supplements (e.g., hydrolysates)] are complex mixtures that are usually comprised of a wide variety of components, including but not limited to salts, amino acids, sugars, vitamins, and minerals. The type and amount of these individual media components are quantitatively formulated to maximize protein expression, yield, and refolding of proteins to the active state.

Cell culture media [which includes basal cell culture media, nutrient feed, and supplements (e.g., hydrolysates)] are complex mixtures that are usually comprised of a wide variety of components, including but not limited to salts, amino acids, sugars, vitamins, and minerals. The type and amount of these individual media components are quantitatively formulated to maximize protein expression, yield, and refolding of proteins to the active state.

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