BRIEFING

 Guayusa Leaf. A new USP monograph for this dietary ingredient is being proposed. The liquid chromatographic method in the test for the Content of Caffeine and Hydroxycinnamoylquinic Acids was conducted using the Waters Acquity BEH Shield RP 18 brand of column with L1 packing. The typical retention times for theobromine, theophylline, neochlorogenic acid, caffeine, cryptochlorogenic acid, 5-O-feruloylquinic acid, chlorogenic acid, 4-O-feruloylquinic acid, isochlorogenic acid C, isochlorogenic acid A, and isochlorogenic acid B were 1.6, 1.9, 2.4, 2.6, 3.6, 3.8, 4.0, 5.4, 6.8, 7.0, and 7.2 min, respectively.

 (BDSHM: M. Monagas)

 Case ID—SUB-203; SUB-2145